Potential Midterm Questions
Popularity (by total correct streak): 0
Popularity (by number of users): 0
| The smallest object that can be resolved by an ordinary light microscope has a diameter of about: | 0.2 microns | |
| The magnification of a microscope: | is equal to the product of the magnification of the objective and the ocular | |
| Immersion oil is used with high magnification because it: | increases the resolving power of the microscope | |
| In dark-field microscopy specimens are viewed by: | reflected light | |
| An image cannot be seen directly by the human eye through | ultraviolet and electron microscopes | |
| An advantage of the phase-contrast microscope is that it: | renders visible the cellular detail of living, unstained cells | |
| Magnification is obtained by: | multiplying the eyepiece (ex. 10x) by the objective (ex. 1.8 mm). | |
| The magnification which you would obtain using a 10x eyepiece (ocular) and a 1.8 mm objective is: | 1000x | |
| The part of the microscope that should be adjusted in order to regulate the amount of light is the: | diaphragm | |
| The changing of objectives without major focusing adjustments is called: | parfocal | |
| The meter is made up of how many micrometers (µm)? | 1,000,000 µm | |
| A micrometer is how many mm? | 1/1000 mm | |
| How many mL are in 1 fluid oz.? | 29.57 mL | |
| How many grams are in a pound? | 453.6 grams | |
| A certain drug is to be administered. The physician's order is for 50 mg, but the available vial is marked | 5 cc | |
| What are hanging drop preparations? | Organisms are mounted in water in a small, draft-free chamber in which they cannot be influenced by air currents. | |
| Hanging drop preparations are especially good for determining: | motility | |
| An organism that retains the crystal violet-iodine complex even when rinsed with acetone-alcohol or some corresponding decolorizer is: | gram positive | |
| Gram-positive bacteria generally: | have low lipid content in their cell walls and are susceptible to penicillin | |
| You obtain a clean slide, put a drop of water on it, scrape some tissue off your cheek, dab it in the water and put a cover on it. You have just prepared what is known as a: | wet mount | |
| A gram-positive organism is distinguished from a gram-negative organism by: | taking up the purple stain and resisting decolorization | |
| The acid fast stain is used for identification of: | tuberculosis | |
| The correct order of adding reagents in a gram stain is: | crystal violet, grams iodine, acetone/alcohol, safranin | |
| When properly stained, gram-positive cells turn: | purple | |
| The gram stain is a: | differential stain | |
| What is the most critical step in the gram-stain? | decolorization | |
| An organism that obtains energy from oxidation of glucose is a: | chemoorganotroph | |
| Photolithotrophs and chemolithotrophs are generally: | autotrophs | |
| Elements essential for cellular metabolism are: | carbon, hydrogen, nitrogen, oxygen | |
| Bacteria cultivated in the laboratory must be provided with: | water, a nitrogen source, and a carbon source | |
| Heterotrophs are most conveniently cultivated on: | complex medium | |
| An example of a complex medium is: | glucose, peptone, and yeast extract | |
| The most commonly used substance for solidifying media is: | agar | |
| A pure culture is defined as one that: | contains organisms of only one species | |
| The small portion of an old culture that is transferred to fresh media to start a new culture is called an: | inoculum | |
| Bacteria that grow well at temperatures near 0ºC are known as: | psychrophiles | |
| How many pounds are in a kilogram? | 2.2 lbs | |
| What is the TSI result for Pseudomonas? | K/K | |
| Is Clostridium an aerobe or an anaerobe? | An anaerobe | |
| Positive result for glucose: | yellow | |
| Negative result for glucose: | red | |
| Positive result for Lysine: | purple | |
| Negative result for Lysine: | tan | |
| Positive result for Ornithine: | purple | |
| Negative result for Ornithine: | tan | |
| Positive result for H2S: | black | |
| Negative result for H2S: | tan | |
| Positive result for Indole: | red | |
| Negative result for Indole: | tan | |
| Positive result for Adonitol: | yellow | |
| Negative result for Adonitol: | red | |
| Positive result for Lactose: | yellow | |
| Negative result for Lactose: | red | |
| Positive result for Arabinose: | yellow | |
| Negative result for Arabinose: | red | |
| Positive result for Sorbitol: | yellow | |
| Negative result for Sorbitol: | red | |
| Positive result for VP: | tan/red | |
| Negative result for VP: | cream | |
| Positive result for Dulcitol: | yellow | |
| Negative result for Dulcitol: | green | |
| Positive result for Phenylalanine: | brown | |
| Negative result for Phenylalanine: | green | |
| Positive result for Urea: | pink | |
| Negative result for Urea: | tan | |
| Positive result for Citrate: | purple | |
| Negative result for Citrate: | green | |
| Lysine is also known as: | LDC | |
| Ornithine is also known as: | ODC | |
| What color is Serratia? | red | |
| Positive Decarboxylase reaction: | purple | |
| Bacteria that grow well at temperatures near 0ºC are known as: | psychrophiles | |
| Bacteria that grow well at temperatures near 20-40ºC are known as: | mesophiles | |
| Bacteria that grow well at temperatures near 50-60ºC are known as: | thermophiles | |
| Sterilization is best achieved by: | autoclaving for 20 minutes | |
| In which of these ways does autoclaving kill microorganisms? | coagulation of cellular proteins | |
| Sterilization is: | being free of all life | |
| A sterilizing oven is best set at: | 165-170ºC | |
| The time required for sterilization or disinfectant may be affected by: | the kind of organism to be killed, the nature of the agent used, pH, extraneous organic material | |
| Autoclaving and filtration are methods of: | sterilization | |
| Sterilization of dry glassware is best done by: | hot air sterilization | |
| A compound designated as a disinfectant should: | destroy or inhibit pathogens | |
| The word used for an agent that will kill bacteria is: | bactericidal | |
| Pasteurization is best accomplished at: | 71.7ºC for 15 seconds or at 62.8ºC for 30 minutes | |
| Disinfectants kill: | pathogens | |
| Iodine compounds are used primarily: | as a skin antiseptic | |
| Alcohol is a good disinfectant at which concentration? | 50-70% | |
| The actions of disinfectants are affected by: | the presence of organic matter | |
| The standard for disinfectants: | phenol | |
| Specific biochemical reactions are blocked by: | antimetabolites | |
| Aniline dyes (such as crystal violet) are effective against: | gram-positive bacteria | |
| Two compounds used in gaseous form for sterilization are: | ethylene oxide and beta-propiolactone | |
| Chemical compounds highly toxic to microorganisms include: | halogens and heavy metals | |
| Hydrogen peroxide is used primarily against: | anaerobes |
Quisition is a browser-based flashcard system that repeats old cards and introduces new ones at optimal time intervals. You can create your own card packs or use those developed by others.
